Evaluation of effect during cell isolation process in alkaline comet assay using epidermal skin cells
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چکیده
منابع مشابه
Improvement of single-cell gel electrophoresis (SCGE) alkaline comet assay
Although the single-cell gel electrophoresis (SCGE) alkaline comet assay can detect DNA damage quickly and with high sensitivity, it does not work well for cryopreserved sperm of some marine teleosts. Using large yellow croaker Pseudosciaena crocea sperm that were cryopreserved in increasing concentrations (5 to 30%) of DMSO, we made modifications to the classic SCGE method that included using ...
متن کاملEvaluation of in vivo genotoxicity of cypermethrin in Drosophila melanogaster using the alkaline Comet assay.
The single cell gel electrophoresis (SCGE) assay, also known as the Comet assay, is one of the most promising genotoxicity tests developed in recent years to measure and analyse DNA damage in single cells. The present study was undertaken to assess the in vivo genotoxicity of the synthetic pyrethroid cypermethrin in brain ganglia and anterior mid gut of Drosophila melanogaster. Freshly emerged ...
متن کاملEvaluation of DNA damage in radiotherapy-treated cancer patients using the alkaline comet assay.
The aim of this study was to evaluate primary DNA damage and the dynamics of the repair of radiotherapy-induced DNA lesions in non-target cells of cancer patients. This study included patients diagnosed with different solid tumors who received radiotherapy. The levels of DNA damage were evaluated using the alkaline comet assay on peripheral blood leukocytes. Altogether four blood samples per pa...
متن کاملRecommendations for conducting the in vivo alkaline Comet assay. 4th International Comet Assay Workshop.
The in vivo alkaline single cell gel electrophoresis assay, hereafter the Comet assay, can be used to investigate the genotoxicity of industrial chemicals, biocides, agrochemicals and pharmaceuticals. The major advantages of this assay include the relative ease of application to any tissue of interest, the detection of multiple classes of DNA damage and the generation of data at the level of th...
متن کاملA Study of Cytogenetic Stability of Induced Pluripotent Stem Cells Using Karyotyping and Comet Assay Techniques
Background & Aims: Induced pluripotent stem cells (iPSCs) have the capability to undergo unlimited selfrenewal and differentiation into all cell types in the body. These cells are artificially derived from a nonpluripotent cell, typically human dermal fibroblasts (HDFs). The study of cytogenetic stability of these cells, in order to use iPS cells and apply studies in therapeutic applications, i...
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ژورنال
عنوان ژورنال: The Journal of Toxicological Sciences
سال: 2012
ISSN: 0388-1350,1880-3989
DOI: 10.2131/jts.37.1267